LOCUS       40924_23754       12570 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Gateway binary vector pGWB721 DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12570)
  AUTHORS   Tanaka Y, Nakamura S, Kawamukai M, Koizumi N, Nakagawa T.
  TITLE     Development of a series of gateway binary vectors possessing a 
            tunicamycin resistance gene as a marker for the transformation of 
            Arabidopsis thaliana
  JOURNAL   Biosci. Biotechnol. Biochem. 75 (4), 804-807 (2011)
  PUBMED    21512216
REFERENCE   2  (bases 1 to 12570)
  AUTHORS   Tanaka Y, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane 
            University, Center for Integrated Research in Science; 1060 
            Nishikawatsu, Matsue, Shimane 690-8504, Japan
REFERENCE   3  (bases 1 to 12570)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 12570)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biosci. 
            Biotechnol. Biochem."; date: "2011"; volume: "75"; issue: "4"; 
            pages: "804-807"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (12-JAN-2011) Contact:Tsuyoshi Nakagawa Shimane University, Center 
            for Integrated Research in Science; 1060 Nishikawatsu, Matsue, 
            Shimane 690-8504, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     constructed using pPZP221.
FEATURES             Location/Qualifiers
     source          1..12570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(54..78)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        812..1157
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     CDS             1193..1222
                     /codon_start=1
                     /label=Myc
                     /note="Myc (human c-Myc proto-oncogene) epitope tag"
                     /translation="EQKLISEEDL"
     CDS             1229..1258
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1274..1303
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1310..1339
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1346..1375
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1391..1420
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1427..1456
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1463..1492
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1508..1537
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1544..1573
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1592..1716
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1753..1783
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1837..2493
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2838..3140
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(3184..3308)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      3334..3586
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3620..3636)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3644..3660
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3668..3698)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3713..3734)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3770..4022)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4060..5355)
                     /codon_start=1
                     /gene="gpt"
                     /product="UDP-N-acetylglucosamine: dolichol phosphate 
                     N-acetylglucosamine-1-P transferase"
                     /label=gpt
                     /protein_id="BAJ61220.1"
                     /translation="MAARKRASSISIPNKPDPSEPNSAPSEQKMTRKTVSASGEEFRLA
                     PPKLGVIFVISTLLCSLYLYLLCFHYKVDNELKRSILINAGLSLVGFFVTLKMIPVTAR
                     YVLRRNMFGFDINKRGTPQGDIKVPESLGIVVGIVFLIVAIIFQYFNFTEDSNWLVEYN
                     AALASICFMILLGFVDDVLDVPWRVKLVLPSFATLPLLMAYAGHTTIVIPKPLVAYIGL
                     EVLNLGRIYKLYMGLLAVFCTNSINIHAGLNGLEIGQTVVIAAAILIHNVMQIGASVDP
                     EYHQAHAFSIFLTQPLMATSLAMLAYNWYPSSVFVGDTYTVFAGMTMAVVGILGHFSET
                     LLIFFLPQVLNLLLSLPQLAGIVKCPRHRLPRYDPATGLLTGTKDGTLVNVYLRLFGPK
                     SEKSLCIHLLVFQALACAFCFILRHFLAGWYK"
     gene            complement(4060..5355)
                     /gene="gpt"
                     /label=gpt
     promoter        complement(5407..5586)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(6092..6116)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6637..7425
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7674..8262
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8448..8588)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8932..9126)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9195..10265)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="VSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10697..11323)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
      961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1141 ttcatttgga gagaacacgg gggactctag aatgagcggg ttaattaacg gtgaacaaaa
     1201 gctaatctcc gaggaagact tgaacggtga acaaaaatta atctcagaag aagacttgaa
     1261 cggactcgac ggtgaacaaa agttgatttc tgaagaagat ttgaacggtg aacaaaagct
     1321 aatctccgag gaagacttga acggtgaaca aaaattaatc tcagaagaag acttgaacgg
     1381 actcgacggt gaacaaaagt tgatttctga agaagatttg aacggtgaac aaaagctaat
     1441 ctccgaggaa gacttgaacg gtgaacaaaa attaatctca gaagaagact tgaacggact
     1501 cgacggtgaa caaaagttga tttctgaaga agatttgaac ggtgaacaaa agctaatctc
     1561 cgaggaagac ttgaacggta gcgctgttat cacaagtttg tacaaaaaag ctgaacgaga
     1621 aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac
     1681 ataatactgt aaaacacaac atatccagtc actatggcgg cccctcgagg ggccgcatta
     1741 ggcaccccag gctttacact ttatgcttcc ggctcgtata atgtgtggat tttgagttag
     1801 gatccgtcga gattttcagg agctaaggaa gctaaaatgg agaaaaaaat cactggatat
     1861 accaccgttg atatatccca atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt
     1921 gctcaatgta cctataacca gaccgttcag ctggatatta cggccttttt aaagaccgta
     1981 aagaaaaata agcacaagtt ttatccggcc tttattcaca ttcttgcccg cctgatgaat
     2041 gctcatccgg aattccgtat ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt
     2101 cacccttgtt acaccgtttt ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa
     2161 taccacgacg atttccggca gtttctacac atatattcgc aagatgtggc gtgttacggt
     2221 gaaaacctgg cctatttccc taaagggttt attgagaata tgtttttcgt ctcagccaat
     2281 ccctgggtga gtttcaccag ttttgattta aacgtggcca atatggacaa cttcttcgcc
     2341 cccgttttca ccatgggcaa atattatacg caaggcgaca aggtgctgat gccgctggcg
     2401 attcaggttc atcatgccgt ttgtgatggc ttccatgtcg gcagaatgct taatgaatta
     2461 caacagtact gcgatgagtg gcagggcggg gcgtaaacgc gtggatccgg cttactaaaa
     2521 gccagataac agtatgcgta tttgcgcgct gatttttgcg gtataagaat atatactgat
     2581 atgtataccc gaagtatgtc aaaaagaggt atgctatgaa gcagcgtatt acagtgacag
     2641 ttgacagcga cagctatcag ttgctcaagg catatatgat gtcaatatct ccggtctggt
     2701 aagcacaacc atgcagaatg aagcccgtcg tctgcgtgcc gaacgctgga aagcggaaaa
     2761 tcaggaaggg atggctgagg tcgcccggtt tattgaaatg aacggctctt ttgctgacga
     2821 gaacaggggc tggtgaaatg cagtttaagg tttacaccta taaaagagag agccgttatc
     2881 gtctgtttgt ggatgtacag agtgatatta ttgacacgcc cgggcgacgg atggtgatcc
     2941 ccctggccag tgcacgtctg ctgtcagata aagtctcccg tgaactttac ccggtggtgc
     3001 atatcgggga tgaaagctgg cgcatgatga ccaccgatat ggccagtgtg ccggtctccg
     3061 ttatcgggga agaagtggct gatctcagcc accgcgaaaa tgacatcaaa aacgccatta
     3121 acctgatgtt ctggggaata taaatgtcag gctcccttat acacagccag tctgcaggtc
     3181 gaccatagtg actggatatg ttgtgtttta cagtattatg tagtctgttt tttatgcaaa
     3241 atctaattta atatattgat atttatatca ttttacgttt ctcgttcagc tttcttgtac
     3301 aaagtggtga taacctagag ctcgaatttc cccgatcgtt caaacatttg gcaataaagt
     3361 ttcttaagat tgaatcctgt tgccggtctt gcgatgatta tcatataatt tctgttgaat
     3421 tacgttaagc atgtaataat taacatgtaa tgcatgacgt tatttatgag atgggttttt
     3481 atgattagag tcccgcaatt atacatttaa tacgcgatag aaaacaaaat atagcgcgca
     3541 aactaggata aattatcgcg cgcggtgtca tctatgttac tagatcggga attggttccg
     3601 gaaccaattc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     3661 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     3721 gctaactcac attaggctga attaggcgcg cctatttcta ttaattcccg atctagtaac
     3781 atagatgaca ccgcgcgcga taatttatcc tagtttgcgc gctatatttt gttttctatc
     3841 gcgtattaaa tgtataattg cgggactcta atcataaaaa cccatctcat aaataacgtc
     3901 atgcattaca tgttaattat tacatgctta acgtaattca acagaaatta tatgataatc
     3961 atcgcaagac cggcaacagg attcaatctt aagaaacttt attgccaaat gtttgaacga
     4021 tcggggaaat tcgcctcgag tgacttctct gattgcagac tatttatacc aaccagcaag
     4081 aaagtgtcga agtatgaagc agaaggcgca ggctagagcc tggaaaacga gaagatggat
     4141 gcaaagagac ttttctgatt taggaccaaa caacctcaag taaacattga cgagtgtccc
     4201 atcttttgtg ccggtcagta atccggtagc aggatcatac cttgggagac gatggcgcgg
     4261 acatttcaca atcccagcaa gctgaggaag cgataataga aggttcagaa cttgaggaag
     4321 aaagaagatg aggagggttt cactgaagtg acccagaata ccaacaactg ccatagtcat
     4381 tccggcgaag actgtgtaag tgtctccaac aaaaactgaa gaaggatacc aattgtaagc
     4441 aagcattgcc aaggatgtgg ccatcagcgg ttgagtaaga aatatcgaga aggcatgagc
     4501 ttggtgatac tcgggatcaa cagatgctcc gatttgcata acgttgtgta tcaggatagc
     4561 agcggcaata acaactgttt gaccgatttc aaggccattc agaccagcgt ggatgttaat
     4621 agagtttgta caaaagacag caagtagccc catatataac ttatatatcc ttcctagatt
     4681 aagcacttcc aagccaatat aagcaacaag aggttttggt ataacaatag ttgtatgacc
     4741 agcataagcc atcaaaagtg gaagggtagc aaaagatggc aggacaagtt ttactctcca
     4801 aggcacatca aggacatcat ctacaaatcc aagcaaaatc atgaagcaaa tagatgctag
     4861 tgctgcatta tactccacaa gccaattaga atcttcagtg aagttaaaat actgaaatat
     4921 tatcgccacg atcaaaaaga caataccaac aacaataccc aatgactctg gcactttaat
     4981 atctccttga ggcgtgcctc ttttgttgat atcaaaacca aacatgttac gtctcagaac
     5041 gtatctggca gtaacaggaa tcattttgag agtgacgaaa aacccaacca agctaagccc
     5101 cgcgttaatt agaatcgatc gcttgagttc attatcaacc ttgtagtgaa aacagagaag
     5161 gtatagatag agcgaacaga gaagagtaga gatcacgaag atcactccta atttcggcgg
     5221 cgcgagacga aattcctcac cggaagcaga aaccgtcttt cgggtcatct tctgctccga
     5281 tggcgctgag ttgggttccg acgggtccgg tttgttggga attgagatcg atgaagctct
     5341 cttacgagct gccatatctt attgcccccc gggatcagat ctggattgag agtgaatatg
     5401 agactctaat tggataccga ggggaattta tggaacgtca gtggagcatt tttgacaaga
     5461 aatatttgct agctgatagt gaccttaggc gacttttgaa cgcgcaataa tggtttctga
     5521 cgtatgtgct tagctcatta aactccagaa acccgcggct gagtggctcc ttcaacgttg
     5581 cggttctgtc agttccaaac gtaaaacggc ttgtcccgcg tcatcggcgg gggtcataac
     5641 gtgactccct taattctccg ctcatgatct tgatcccctg cgccatcaga tccttggcgg
     5701 caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc
     5761 tggcaattcc ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag
     5821 cccactgcaa gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca
     5881 gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtgttccg
     5941 cttcctttag cagcccttgc gccctgagtg cttgcggcag cgtgaagctg atccgtcgag
     6001 attaatagaa ataaattcag cctaattcgg cgttaattca gtacattaaa aacgtccgca
     6061 atgtgttatt aagttgtcta agcgtcaatt tgtttacacc acaatatatc ctgccaccag
     6121 ccagccaaca gctccccgac cggcagctcg gcacaaaatc accactcgat acaggcagcc
     6181 catcagtccg ggacggcgtc agcgggagag ccgttgtaag gcggcagact ttgctcatgt
     6241 taccgatgct attcggaaga acggcaacta agctgccggg tttgaaacac ggatgatctc
     6301 gcggagggta gcatgttgat tgtaacgatg acagagcgtt gctgcctgtg atcaattcgg
     6361 gcacgaaccc agtggacata agcctgttcg gttcgtaagc tgtaatgcaa gtagcgtatg
     6421 cgctcacgca actggtccag aaccttgacc gaacgcagcg gtggtaacgg cgcagtggcg
     6481 gttttcatgg cttgttatga ctgttttttt ggggtacagt ctatgcctcg ggcatccaag
     6541 cagcaagcgc gttacgccgt gggtcgatgt ttgatgttat ggagcagcaa cgatgttacg
     6601 cagcagggca gtcgccctaa aacaaagtta aacatcatgg gggaagcggt gatcgccgaa
     6661 gtatcgactc aactatcaga ggtagttggc gtcatcgagc gccatctcga accgacgttg
     6721 ctggccgtac atttgtacgg ctccgcagtg gatggcggcc tgaagccaca cagtgatatt
     6781 gatttgctgg ttacggtgac cgtaaggctt gatgaaacaa cgcggcgagc tttgatcaac
     6841 gaccttttgg aaacttcggc ttcccctgga gagagcgaga ttctccgcgc tgtagaagtc
     6901 accattgttg tgcacgacga catcattccg tggcgttatc cagctaagcg cgaactgcaa
     6961 tttggagaat ggcagcgcaa tgacattctt gcaggtatct tcgagccagc cacgatcgac
     7021 attgatctgg ctatcttgct gacaaaagca agagaacata gcgttgcctt ggtaggtcca
     7081 gcggcggagg aactctttga tccggttcct gaacaggatc tatttgaggc gctaaatgaa
     7141 accttaacgc tatggaactc gccgcccgac tgggctggcg atgagcgaaa tgtagtgctt
     7201 acgttgtccc gcatttggta cagcgcagta accggcaaaa tcgcgccgaa ggatgtcgct
     7261 gccgactggg caatggagcg cctgccggcc cagtatcagc ccgtcatact tgaagctaga
     7321 caggcttatc ttggacaaga agaagatcgc ttggcctcgc gcgcagatca gttggaagaa
     7381 tttgtccact acgtgaaagg cgagatcacc aaggtagtcg gcaaataatg tctagctaga
     7441 aattcgttca agccgacgcc gcttcgcggc gcggcttaac tcaagcgtta gatgcactaa
     7501 gcacataatt gctcacagcc aaactatcag gtcaagtctg cttttattat ttttaagcgt
     7561 gcataataag ccctacacaa attgggagat atatcatgca tgaccaaaat cccttaacgt
     7621 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     7681 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     7741 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     7801 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     7861 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     7921 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     7981 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     8041 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     8101 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     8161 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     8221 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     8281 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     8341 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     8401 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat
     8461 tttctcctta cgcatctgtg cggtatttca caccgcatat ggtgcactct cagtacaatc
     8521 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca
     8581 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     8641 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     8701 caccgtcatc accgaaacgc gcgaggcagg gtgccttgat gtgggcgccg gcggtcgagt
     8761 ggcgacggcg cggcttgtcc gcgccctggt agattgcctg gccgtaggcc agccattttt
     8821 gagcggccag cggccgcgat aggccgacgc gaagcggcgg ggcgtaggga gcgcagcgac
     8881 cgaagggtag gcgctttttg cagctcttcg gctgtgcgct ggccagacag ttatgcacag
     8941 gccaggcggg ttttaagagt tttaataagt tttaaagagt tttaggcgga aaaatcgcct
     9001 tttttctctt ttatatcagt cacttacatg tgtgaccggt tcccaatgta cggctttggg
     9061 ttcccaatgt acgggttccg gttcccaatg tacggctttg ggttcccaat gtacgtgcta
     9121 tccacaggaa agagaccttt tcgacctttt tcccctgcta gggcaatttg ccctagcatc
     9181 tgctccgtac attaggaacc ggcggatgct tcgccctcga tcaggttgcg gtagcgcatg
     9241 actaggatcg ggccagcctg ccccgcctcc tccttcaaat cgtactccgg caggtcattt
     9301 gacccgatca gcttgcgcac ggtgaaacag aacttcttga actctccggc gctgccactg
     9361 cgttcgtaga tcgtcttgaa caaccatctg gcttctgcct tgcctgcggc gcggcgtgcc
     9421 aggcggtaga gaaaacggcc gatgccggga tcgatcaaaa agtaatcggg gtgaaccgtc
     9481 agcacgtccg ggttcttgcc ttctgtgatc tcgcggtaca tccaatcagc tagctcgatc
     9541 tcgatgtact ccggccgccc ggtttcgctc tttacgatct tgtagcggct aatcaaggct
     9601 tcaccctcgg ataccgtcac caggcggccg ttcttggcct tcttcgtacg ctgcatggca
     9661 acgtgcgtgg tgtttaaccg aatgcaggtt tctaccaggt cgtctttctg ctttccgcca
     9721 tcggctcgcc ggcagaactt gagtacgtcc gcaacgtgtg gacggaacac gcggccgggc
     9781 ttgtctccct tcccttcccg gtatcggttc atggattcgg ttagatggga aaccgccatc
     9841 agtaccaggt cgtaatccca cacactggcc atgccggccg gccctgcgga aacctctacg
     9901 tgcccgtctg gaagctcgta gcggatcacc tcgccagctc gtcggtcacg cttcgacaga
     9961 cggaaaacgg ccacgtccat gatgctgcga ctatcgcggg tgcccacgtc atagagcatc
    10021 ggaacgaaaa aatctggttg ctcgtcgccc ttgggcggct tcctaatcga cggcgcaccg
    10081 gctgccggcg gttgccggga ttctttgcgg attcgatcag cggccgcttg ccacgattca
    10141 ccggggcgtg cttctgcctc gatgcgttgc cgctgggcgg cctgcgcggc cttcaacttc
    10201 tccaccaggt catcacccag cgccgcgccg atttgtaccg ggccggatgg tttgcgaccg
    10261 ctcacgccga ttcctcgggc ttgggggttc cagtgccatt gcagggccgg cagacaaccc
    10321 agccgcttac gcctggccaa ccgcccgttc ctccacacat ggggcattcc acggcgtcgg
    10381 tgcctggttg ttcttgattt tccatgccgc ctcctttagc cgctaaaatt catctactca
    10441 tttattcatt tgctcattta ctctggtagc tgcgcgatgt attcagatag cagctcggta
    10501 atggtcttgc cttggcgtac cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac
    10561 tgaaagttga cccgcttcat ggctggcgtg tctgccaggc tggccaacgt tgcagccttg
    10621 ctgctgcgtg cgctcggacg gccggcactt agcgtgtttg tgcttttgct cattttctct
    10681 ttacctcatt aactcaaatg agttttgatt taatttcagc ggccagcgcc tggacctcgc
    10741 gggcagcgtc gccctcgggt tctgattcaa gaacggttgt gccggcggcg gcagtgcctg
    10801 ggtagctcac gcgctgcgtg atacgggact caagaatggg cagctcgtac ccggccagcg
    10861 cctcggcaac ctcaccgccg atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg
    10921 cttgtagcct tccatccgtg acctcaatgc gctgcttaac cagctccacc aggtcggcgg
    10981 tggcccatat gtcgtaaggg cttggctgca ccggaatcag cacgaagtcg gctgccttga
    11041 tcgcggacac agccaagtcc gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc
    11101 ggccgatggc cttcacgtcg cggtcaatcg tcgggcggtc gatgccgaca acggttagcg
    11161 gttgatcttc ccgcacggcc gcccaatcgc gggcactgcc ctggggatcg gaatcgacta
    11221 acagaacatc ggccccggcg agttgcaggg cgcgggctag atgggttgcg atggtcgtct
    11281 tgcctgaccc gcctttctgg ttaagtacag cgataacctt catgcgttcc ccttgcgtat
    11341 ttgtttattt actcatcgca tcatatacgc agcgaccgca tgacgcaagc tgttttactc
    11401 aaatacacat caccttttta gacggcggcg ctcggtttct tcagcggcca agctggccgg
    11461 ccaggccgcc agcttggcat cagacaaacc ggccaggatt tcatgcagcc gcacggttga
    11521 gacgtgcgcg ggcggctcga acacgtaccc ggccgcgatc atctccgcct cgatctcttc
    11581 ggtaatgaaa aacggttcgt cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg
    11641 cgttcattct cggcggccgc cagggcgtcg gcctcggtca atgcgtcctc acggaaggca
    11701 ccgcgccgcc tggcctcggt gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg
    11761 gtcgagcgat gcacgccaag cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg
    11821 atcagctcgc gggcgtgcgc gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc
    11881 acgcctcggg ccttggcggc ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc
    11941 tcgaactcgg caatgccggc gaacacggtc aacaccatgc ggccggccgg cgtggtggtg
    12001 tcggcccacg gctctgccag gctacgcagg cccgcgccgg cctcctggat gcgctcggca
    12061 atgtccagta ggtcgcgggt gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg
    12121 tcgccagggc gtaggtggtc aagcatcctg gccagctccg ggcggtcgcg cctggtgccg
    12181 gtgatcttct cggaaaacag cttggtgcag ccggccgcgt gcagttcggc ccgttggttg
    12241 gtcaagtcct ggtcgtcggt gctgacgcgg gcatagccca gcaggccagc ggcggcgctc
    12301 ttgttcatgg cgtaatgtct ccggttctag tcgcaagtat tctactttat gcgactaaaa
    12361 cacgcgacaa gaaaacgcca ggaaaagggc agggcggcag cctgtcgcgt aacttaggac
    12421 ttgtgcgaca tgtcgttttc agaagacggc tgcactgaac gtcagaagcc gactgcacta
    12481 tagcagcgga ggggttggat caaagtactt tgatcccgag gggaaccctg tggttggcat
    12541 gcacatacaa atggacgaac ggataaacct
//
